Najmul Islam
Aligarh Muslim University, India
Title: Allicin from garlic having beneficial effects in combating diseases
Biography
Biography: Najmul Islam
Abstract
Introduction & Aim: Th e relationship between Reactive Oxygen Species (ROS) and various diseases like metabolic cardiac disorders, osteoporosis, tuberculosis and cancer and are well documented. Th e present study involves the employment of a natural compound namely allicin from garlic having anti-oxidant and anti-infl ammatory properties with proven health benefi ts. Our preliminary observations appear to possibly provide some scientifi c input that may be useful in the management of Ischemic Heart Disease (IHD), osteoporosis, tuberculosis and cancer. Method: Peripheral Blood Mononuclear Cells (PBMC’s) were isolated by density gradient method from blood of patients with Ischemic Heart Disease (IHD), osteoporosis, tuberculosis and cancer (n=20 each) and were employed in culture studies with and without of varying does of allicin (0-500 ng/ml). Th e 24 hr cultures were probed for CK, sTNF-alpha, sRANKL levels as well as for Glutathione Peroxidase (GPx) activity. Result: Cells were collected aft er 24 hours with and without allicin (0-500 ng/ml; n=20 each). An appreciably suppressed GPx activity was recorded in cell cultures of patient’s with IHD, osteoporosis, tuberculosis and cancer when compared to samples of healthy controls where the GPx data refl ects upon the compromised defense system in patients with Ischemic Heart Disease (IHD). On the contrary, treatment or co-culturing with varying doses of allicin (0-500 ng/ml) exhibited a remarkable degree of amelioration in GPx activity in cells of all the above four types of diseased patients. Next, the ELISA data showed that the 24 hr culture supernatants of untreated patients cells were having augmented expressions of sTNF-alpha, which upon co-culturing with 500 ng/ml of allicin resulted in an appreciable degree of downregulation/suppression in the expressions of sTNF-alpha in cells of all the above four types of diseased patients. Cultures from all patient types exhibited a dose dependent suppression with allicin. Similarly, in IHD patients, in comparison to untreated controls, a dose dependent decrease in CK levels were observed in cultures receiving allicin (0-500 ng/ml; n=10). Also, in cell cultures from osteoporosis patients, allicin (0-500 ng/ ml), showed an appreciable degree of downregulation in sRANKL. Conclusion: Th e encouraging preliminary data suggested that in-depth studies are required at the molecular level, which in turn, may provide information for possibly employing allicin as potential adjunct in the management of Ischemic Heart Disease (IHD), osteoporosis, tuberculosis and cancer. repute.